Composite

Part:BBa_K3482004:Design

Designed by: Thierry Marti   Group: iGEM20_UNILausanne   (2020-10-18)


IPTG-inducible miniColicin toxin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 124
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Growth inhibition can occur even without IPTG induction due to leaky expression of the pTac promoter.


Source

This part is composed of the pTac promoter (BBa_K1934040) and the miniColicin (BBa_K1976048), the cytotoxin domain of the bacterioicin colicin E2.

References